METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
2
Manys studies showed that angiopoietin-1(ANG-1) could stimulate coronary collateral artery formation and angiopoietin-2(ANG-2) might inhibit angiogenesis by inhibition of ANG-1.
目的：本研究拟探讨冠脉严重狭窄患者血清血管生成素-1，血管生成素-2浓度与冠脉侧支形成的关系。
3
The cloning of human angiopoietin1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.