Conclusion The expressive rate can be elevated bychromatography and purification.
结论经过层析和纯化可以提高表达率。
2
Methods:The isolation and purification were carried out by repeated silica gel column chromatography, and the chemical structures were determined by physicochemical properties and spectral analysis.
方法：采用反复硅胶柱色谱、葡聚糖凝胶色谱等分离纯化，通过理化常数和波谱分析鉴定其化学结构。
3
Purpose To study purification method of the low molecular weight heparin(LMWH) by affinity chromatography.