Methods:Polymerase chain reaction (PCR) and singlestrandconformationpolymorphism(SSCP) was used.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
2
Singlestrandconformationpolymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
3
Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and singlestrandconformationpolymorphism.