Methods The distribution of opticcup stem cells in opticcup tissue in 12.5-embryonic-day-old rats was observed by immunohistochemistry.
方法采用免疫组织化学技术，检测视杯干细胞在大鼠尾芽胚（第12 .5天胚龄）视杯组织中的分布；
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Conclusion opticcup of 12.5-embryonic-day-old rats composes of undifferentiated cells, and the stem cells are mainly located in opticcup inner and marginal.
Here we report the dynamic, autonomous formation of the opticcup (retinal primordium) structure from a three-dimensional culture of mouse embryonic stem cell aggregates.