Neuner started two seconds behind leader Kuzmina, based on the results of Saturday's sprint event.
BBC: Magdalena Neuner gains revenge in pursuit
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"I am surprised - I gave it all I had, " said Neuner.
BBC: Anastazia Kuzmina wins biathlon sprint gold
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Six-time world champion Neuner, 23, secured her first Olympic gold by taking control with a flawless display in the first two rounds of prone shooting.