The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
Results At each time point, the content of brain tissue, evans blue(EB) and the expressing quantity of ICAM 1 in LPS group increased significantly compared with group A and NS group(P<0.05).