We observed the development of embryos on day3 afterfertilization, then biopsied after laser-assisted ZP drilling.
受精后第三天观察胚胎发育情况，合适胚胎采用激光打孔法进行活检。
2
Phosphorylase gene expression was localized in tissue sections of rice pistils before and afterfertilization by in situ RNA hybridization.
利用RNA原位杂交技术，对水稻受精前后雌蕊组织切片进行磷酸化酶基因表达的定位。
3
The effects of treatment conditions including heat shock temperature, treatment initiation time afterfertilization and treatment duration on development were investigated.