The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。
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Given the correct copy as a template, the recombination event occurred at the break site and 11 percent of the cells tested had traded one copy of the mutant gene for the correct version.
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.