Objective: To develop a simple and efficient way to perform site-directedmutagenesis.
目的:介绍一种简便、有效的定点突变技术。
2
Site-directedmutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
3
The reliability of the method was confirmed by site-directedmutagenesis, selective chemical modification, site-specific antipeptide antibody binding experiments, and so on.