Site-directedmutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
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Objective: To develop a simple and efficient way to perform site-directedmutagenesis.
目的:介绍一种简便、有效的定点突变技术。
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AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) sitedirectedmutagenesis technology.