The interaction between the metal chelate ligand and proteins and the selectivity of metal chelate chromatography can be changed through changing chromatographic conditions.
改变色谱条件可改变金属螯合配体和蛋白质间的作用力，提高金属螯合色谱的选择性。
2
The interaction between chelating weak cation-exchanger and metal ions was examined. The retention mechanism of protein in metal chelating chromatography was explored.
考察了螯合型弱阳离子交换剂与金属离子的作用，研究了蛋白质在金属螯合色谱中的保留机理。
3
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.