The specimens were obtained sterilely with preservative-free heparin, and the cord blood mononuclear cell was isolated by lymphocyteseparationmedium.
无菌条件下取正常足月剖宫产胎儿的脐带血，经肝素抗凝，用淋巴细胞分离液分离脐血单个核细胞。
2
Lymphocyteseparationmedium was used to isolate the mononuclear cells, and the attachment growth method was used to purify endothelial progenitor cells.