ATP-Bioluminescence method allowed the rapid quantification of microorganisms by measuring their intracellular ATP in the firefly luciferin-luciferase BL reaction.
生物发光法是一种用于分析待检测样品中有无微生物及其数量的方法。
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Researchers realised that adding luciferase and luciferin to a sample of water or food could be used to detect ATP, and thus determine whether the sample contained bacteria or other pathogens.
The simplest light-producing reactions require an organic molecule, known as a luciferin, to be oxidised by oxygen, a process that is helped along by an enzyme known as luciferase.