The yeast three-hybrid have three components: a protein fused with DNA binding domain, a hybrid RNA molecule, a protein fused with DNA activationdomain.
酵母三杂交系统由三部分组成：DNA结合结构域融合蛋白、杂交RNA分子、DNA激活结构域融合蛋白。
2
It is good practice to define and register domain roles in the activation phase of a business component.
在业务组件的激活阶段定义和注册域角色是一个很好的练习。
3
Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).