Objective:To explore whether detecting minimal residual disease of leukemia with hyper-methylation of the calcitonin gene as molecular genetic marker of leukemic clone to predict the prognosis.
The results established the foundation of Marker Assisted Selection(MAS) of breeding , the two opposite genes Map-based clone and function study of the gene.
The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.