METHODS 3,5-di-tert-butyl-4-hydroxybenzaldehyde was synthesized from 2,6-di -tert-butyl phenol in a reaction medium of acetic acid-water which its ratio was investigated.
The inhibition effect of 2-hydroxy-4-methoxy-benzaldehyde(HMB)on the activity of monophenolase and diphenolase contained in tyrosinase was studied using enzymological kinetic method.