In this dissertation we focused on the function of genes associated with anther and pollen development including map-based cloning of Arabidopsis genes from male sterile mutants.

However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.

The map makes effective tools for QTL analysis, marker-assisted selection (MAS) in breeding programs and cloning of the genes responsible for various phenotypes (map-based cloning).