Numerical simulations of micro scanning forces and micro topography are presented to investigate the influence of the coupling deformation of AFM probe under the AFM contact mode.

These sections are dissected, using a laser, into slices a millimetre or less thick, and then scanned again, either with the micro-CT or with a scanning electron microscope.

Colour-enhanced scanning electron micro-graph of the split end of a human hair showing the outer cuticle layer surrounding the inner cortical layer.