By growing the mouse mammary tumor derived cell line GR2H6 in 96 well plates, we have developed an in vitro bioassay for the growth promoting activities of insulin.

Mammary carcinoma is a common malignant tumor in women. Identification in early stage is critical to treatment and prognosis of the malignancy.

Conclusions the prognosis of bilateral primary mammary cancer is decided by whether axilla lymph node has transferred, whether the tumor is big or small, and whether the er receptor is positive.