With the beginning of study about the origin of computer graphic design, I specifically analyze the value of the combination of new medium and plane design so that such theory could support design.

The present paper take Canada as an example, introduces the Canadian medium self-regulation historical origin and the theory origin.

The type of callus was an important factor affecting whole plantlets differentiation, and there was a relation between plantlets differentiation and organ origin of callus, as well as medium type.