The use of temperature and acid concentration to increase the rate of sulfonation while controlling the side reaction of sulfone bridging minimizes reaction time while maximizing bead quality.
在控制砜桥副反应的同时，使用能够提高磺化速率的温度和酸浓度，使珠粒质量最佳化的同时降低了反应时间。
2
This part includes preparation of total protein samples from the culture cells, on-bead immuno-binding reaction and wash step, and choice of sampling.