The cell culture procedures take about 4-5 weeks, and cells engrafted in the mouse liver may be detected 2-3 months after transplantation.

All the 60 liver, 80 cell culture and 60 serum samples were correctly diagnosed in good agreement with MCF.

Conclusion The hollow fiber bioreactor for culturing pig hepatocytes can. be prepared by artificial capillary cell culture system, which provides a certain liver-specific function in 1 week.