A novel method for protein purification was presented, which was the integration of organic antisolvent and compressed gas isoelectric precipitation.
将有机溶剂抗溶剂与高压气体等电沉淀技术结合起来,提出了一种新的蛋白质纯化方法。
2
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
定量后,分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
3
It is reported that a comparison with the different of extracting parasporal Crystal Protein of Bacillus thuringiensis between the liquid double-phase method and Isoelectric point method.