The kit comprises the following main components: buffer solution, coenzyme, pyruvic acid, ammonia chloride, pyruvate oxidase, glycine oxidase, alaninedehydrogenase and a stabilizer.
Cu-MMT could destroy bacterial cellular membrane and then resulted in leakage of intracellular enzymes such as asparate aminotransferase, lactate dehydrogenase and alanine aminotransferase.