Affinity chromatography and ionexchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
2
Isolation of the crude extract the crude extract were isolated into two parts, the negative-charged and the positive-charged, by means of Ion-exchange chromatography.