Methods The peptide was separated by ionexchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
2
In this paper, an ion - exchangeHPLC method for the separation and determination of phosphate and phosphite in waste water of electrodes plating of nickel had been built up.