Method: RRTP-1 was obtained by ionexchangecolumnchromatography and gel chromatography.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
2
Methods The peptide was separated by ionexchangecolumnchromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.