Method: RRTP-1 was obtained by ionexchangecolumn chromatography and gel chromatography.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
2
The glycosides in membrane proteins were extracted by using acid and cleared by using ionexchangecolumn.
用酸水解多糖链后经离子交换净化 ，再用醋酐、吡啶加热回流乙酰化。
3
Methods The peptide was separated by ionexchangecolumn chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.