The progress in technique of invitroselection for resistant mutant such as choosing of cultured material, stress factors and mutagens were reviewed in this paper.
For exploiting resistant germplasm, so-maclonal variants were selected from adventitious buds by means of invitroselection using Fusaric Acid(FA) as the selective agent.
In this paper sugarcane callus was cultured invitro on the medium with smut toxin filter solution which was used as selection pressure in this experiment.