Objective: to develop a convenient, inexpensive and efficient method for isolation of functional primary rat hepatocytes, which can be used successfully for invitrometabolism study.
目的：建立一种简便、经济、高效分离原代大鼠肝细胞的方法，适用于体外药物代谢研究。
2
A double wavelength-differential-first order derivative spectrophotometric assay was established to determine carbon monoxide (CO) generation during invitrometabolism of xenobiotics.
用双波长、差示、一阶导数分光光度法，建立了一种测定外源物体外代谢时一氧化碳(CO)生成量的新方法。
3
Aim to investigate the effect of ketoconazole on the metabolisminvitro and pharmacokinetics of saquinavir.