After SDS-PAGE, the recovery of CP added the same volume of incompleteFreundAdjuvant emulsified completely, injected rabbits by subcutaneous injection.
PAGE电泳后，将表达的CP蛋白切胶回收，研磨成粉后加入等体积的福氏不完全佐剂，乳化制备成抗原。
2
The antigen had been emulsified with Freund' s complete adjuvant in the earlier 3 times and with Freund' s incompleteadjuvant in the latter 3 times.