Methods kidneycortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶- EDTA消化传代。
2
The changes of renal cortex microcirculation in rats with burns compained with endotoxemia were observed by making use of the intact hydronephrotic kidney and their related mechanisms were studied.
利用大鼠完整的肾盂积水肾脏，观察了烫伤复合内毒素血症肾皮质微循环的变化，并对其机制进行了初步探讨。
3
Results Benazepril could decreased elevated serum creatinine lever, relative kidney weight and ACE activities in diabetic rats renal cortex.