Although a bucket brigade tried to put it out, helped by new, hand-pump fire engines, the chapel next door caught fire. Both buildings were destroyed.

So if we re-use the same bucket, we lose the links, so that the brigade now jumps over any data between the two instances of it.

Those removals alter electrical charges in a way that allows the protein to pick up other phosphate-containing chemical groups to pass along as part of a molecular bucket brigade.