免疫沉淀
Blood pressure(by Non-invasive Blood Pressure controller instrument),urine protein(by 12-hours urine protein quality),platelet counts,plasma antithrombin activity(by immunoturbidimetry),D-D concentration(by immunoprecipitate sandwich method),thrombin time were tested,body weight,nose-breech length,ponderal index(PI) of fetal mice and placental weight were measured,placental fibrin depositions(by PTAH staining) and thrombomodulin(by IHC staining),pathologies of placentas,livers, kidneys and tails were detected on day 17.5.
分别测定孕鼠血压(无创鼠尾血压测定法)、尿蛋白(12小时尿蛋白浓度测定)、血小板计数、血浆ATⅢ含量(免疫比浊法)、血浆D-D含量(免疫沉淀夹心法)、凝血酶时间;仔鼠体重、鼻臀长、体重指数(PI)和胎盘重量;胎盘纤维蛋白PTAH染色、血栓调节素(TM)免疫组化染色;胎盘、肝、肾、尾HE染色。