Method: Springing to platform test and immunohistochemical ABC were employed.
方法：采用跳台实验和免疫组织化学abc法。
2
Methods By using hemodynamics test, CD34 immunohistochemical method and electron microscope image analysis, 83 patients with varicosity and 12 controls were studied.
Methods The test group and control groups' expression of MMP-2, TIMP-2 were detected by immunohistochemical method, then the ratios of MMP-2/TIMP-2 were calculated.