An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immunesera and other disease sera.
The immune rabbit sera were used for passive immunity test in mice.
并以家兔免疫血清在小鼠进行被动免疫保护实验。
3
The method was proved to be very sensitive, specific and repetitive by detecting different sera samples from clinical and immune ducks, which can diagnose the disease rapidly.