The difference from the gene type of wheat is an importance factor effecting on the one-step culture for immature embryos of wheat.

Wheat genotypes Yangmai 158, Yangmai 10 are excellent transformation materials. Wheat immature embryos with 4 days preculture have high tissue culture response and strong regeneration capacity.

The explants from immature embryo of 4 wheat varieties were cultured in vitro to study the affecting factors on callus induction and differentiation.