The explants from immature embryo of 4 wheat varieties were cultured in vitro to study the affecting factors on callus induction and differentiation.

With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.

After being cultured 50-70 days, embryos (including mature and immature ones) as well as seedlings from the mature embryo are obtained from 7 crosses and 2 selfed cultivars with a certain frequency.