Methods: The gradientelution mode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
方法：采用梯度洗脱的方法进行色谱分离，对各色谱峰进行数据处理，得出红花黄色素的指纹图谱。
2
MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradientelution mode was applied to chromatographic separation.