Objective: (1) to establish PCR reaction system that USES allele-specificprimer PCR technique to detect SNP of KLOTHO gene.
目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
2
Specificprimer PCR was a gene cloning technique with high efficiency, stability and accuracy.
特异引物PCR方法是一种高效、稳定、可靠的基因克隆手段。
3
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specificprimer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.