Methods: the chromosome were prepared with direct method, brief culture of cells and R-banding techniques, and the karyotypicanalysis was performed on the base of complex classification.
方法：采用直接法、短期培养法和R显带技术制备染色体，根据复杂分类法进行染色体核型分析。
2
Conclusion chromosome Karyotypic and Y-chromosome AZF microdeletion analysis are important genetic assays for male spermatogenesis dysfunction.