PCR, nested-PCR and digestion analysis of the Anti-TrxS gene from T1, T2 and T3 plants showed the foreign Anti-TrxS gene was inherited to the progenies.
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果 巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
3
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.