Use a total internal reflection fluorescence microscope to observe, the latter can be sent to filter out the backgroundfluorescence.
这里用全内反射荧光显微镜来进行观察，就可以把后者发出的背景荧光过滤掉。
2
By photon-burst mothod, the fluorescence photons of dye molecules can be distinguished from the background reflecting photons.
通过探测“光子爆发”，把单个染料分子的荧光同很强的背景杂散光区别开来。
3
In order to eliminate or reduces the background or fluorescence influence, it is better to operate in the darkroom or the dark cover screening, to completely eliminate the environment light influence.