The hot water will break down the starch in the grain, turning it into a sugary substance called wort, which is then diluted, boiled, and transferred to a fermentation tank.

Design of a complete set of anaerobic fermentation device. It contents an anaerobic reactor, a constant temperature system by circulating hot water, and a gas collection device.

Through dipping in hot water and ethanol precipitation, a kind of proteinase A inhibitor can be selected from the powder of Ganoderma lucidum by submerged fermentation ;