Step 3: Elute glutathione resin-bound GTPase with SDS buffer.
第三步：使用SDS缓冲液稀释谷胱甘肽树脂。
2
ADP ribosylation factors (ARFs) are critical in the vesicular trafficking pathway. ARF activity is controlled by GTPase-activating proteins (GAPs).
ADP核糖基化因子(ARF)在膜泡运输中具有重要作用，其活性受gtp酶活化蛋白(gap)的调控。
3
Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.