The target proteins were released from the cells by sonication and further purified with GST column.
经超声波破碎菌体释放目蛋白，并用GST亲和层析进一步纯化得到目的蛋白。
2
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.