Because this method was to simulate the process of antibody maturation in vivo, the obtained antibodies was not only fully humanized but also with relatively high affinity.
该方法体外模拟了抗体亲和力成熟的过程，使得到的抗体不但可以完全的人源化，而且可以具有较高的亲和力。
2
We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.