An indirect hemagglutination assay (IHA) was set up using sheep erythrocytes sensitized with capsid protein VP2 of infectious bursal disease virus (IBDV) which were expressed in Escherichia coli.
Methods After amplifing the virus by cell culture, influenza virus was differentiated by using RT-PCR and hemagglutination inhibition test.
方法用常规细胞培养法增殖病毒后，分别用RT-PCR反应法和常规血凝抑制法进行流感病毒鉴定。
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Results: According to hemagglutination titer, it was proved that most of Radix Isatidis and Folium Isatidis were effective to influenza a virus in 3 aspects: direct, therapeutic and preventive action.