B5 as basic medium appended with different concentrations of sucrose, AGAR and different hormone combinations was used to optimize the culture technique for DH line in Brassica napus l.

In adopting tissue culture method, the influence of different explant types, hormone constitutions, sugar concentrations on pepper axillary buds formation and mediums on bud rooting were studied.

The added exogenous hormone and poly amino acid to culture medium can improve the inductivity of embryoid, increasing averagely 59.64% than that of the contrast.