If the tertiary butyloxycarbonyl group is being used as amino-protecting group, its removal is effected with acid, such as HCl and isopropanol as a solvent or with trifluoroacetic acid neat.

Preparation of TFAE through reactive distillation process had been researched, with trifluoroacetic acid and ethanol as raw materials, concentrated sulfuric acid as the catalyst.

Trifluoroacetic acid pyridine salt;